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Removing Lipemia in Serum/Plasma Samples: A Multicenter Study.

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PubMed
Summary

High-speed centrifugation effectively removes lipemia from serum/plasma samples, offering a practical alternative to ultracentrifugation. Liquid-liquid extraction methods like LipoClear and 1,1,2-trichlorotrifluoroethane interfere with biochemical parameter measurements.

Keywords:
1,1,2-trichlorotrifluoroethaneHigh-speed centrifugationInterferenceLipemiaLipid removal methodLipoClear

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Area of Science:

  • Clinical Laboratory Science
  • Analytical Chemistry

Background:

  • Lipemia is a common cause of analytical errors in clinical laboratories.
  • Accurate biochemical parameter measurement requires effective lipemia removal from serum/plasma samples.

Purpose of the Study:

  • To evaluate high-speed centrifugation as a simpler alternative to ultracentrifugation for lipemia removal.
  • To compare high-speed centrifugation with liquid-liquid extraction methods for lipemia removal and their impact on biochemical assays.

Main Methods:

  • Seven Spanish hospital laboratories participated.
  • Compared ultracentrifugation (108,200×g) with high-speed centrifugation (10,000×g for 15 min).
  • Assessed high-speed centrifugation against LipoClear and 1,1,2-trichlorotrifluoroethane, analyzing 14 biochemical parameters for clinically significant interference.

Main Results:

  • No significant interference was found when comparing ultracentrifugation and high-speed centrifugation.
  • Liquid-liquid extraction methods (LipoClear, 1,1,2-trichlorotrifluoroethane) caused clinically significant interference in protein, albumin, and calcium measurements.
  • High-speed centrifugation showed no significant interference for the tested parameters compared to ultracentrifugation.

Conclusions:

  • High-speed centrifugation (10,000×g for 15 min) is a viable and practical alternative to ultracentrifugation for removing lipemia.
  • Liquid-liquid extraction methods are unsuitable due to interference with critical biochemical parameter assays.