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Updated: Feb 7, 2026

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Discharge Plasma-Activated Saline Protects Against Abdominal Sepsis by Promoting Bacterial Clearance

Jingyao Zhang1,2, Kai Qu1, Xia Zhang3

  • 1Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, People's Republic of China.

Shock (Augusta, Ga.)
|July 21, 2018
PubMed
Abstract

Insights

Cold atmospheric discharge plasma-activated saline (DPAS) effectively treats abdominal sepsis in mice by inhibiting bacterial growth and reducing inflammation. This innovative treatment shows significant protective effects against sepsis-induced organ damage and improves survival rates.

Area of Science:

  • Plasma medicine
  • Infectious disease research
  • Sepsis treatment

Background:

  • Abdominal sepsis remains a critical condition with high mortality.
  • Current treatments for sepsis have limitations.
  • Plasma-activated saline (DPAS) is an emerging therapeutic modality.

Purpose of the Study:

  • To investigate the efficacy of cold atmospheric discharge plasma-activated saline (DPAS) in treating abdominal sepsis.
  • To evaluate the in vitro and in vivo effects of DPAS on bacterial proliferation and sepsis progression.
  • To elucidate the underlying mechanisms of DPAS in mitigating sepsis-related pathology.

Main Methods:

  • In vitro: Methicillin-resistant Staphylococcus aureus (MRSA) treated with DPAS.
  • In vivo: Murine model of abdominal sepsis induced by cecal ligation and puncture (CLP).
  • Assessment of survival rates, histopathological injuries, inflammatory markers, bacterial load, and molecular signaling pathways (HMGB1, NF-κB p65).

Main Results:

  • DPAS significantly inhibited MRSA proliferation in vitro.
  • DPAS treatment improved survival rates in a murine sepsis model.
  • DPAS attenuated organ damage, reduced inflammation, decreased bacterial load, and suppressed HMGB1 and NF-κB p65 expression.

Conclusions:

  • DPAS demonstrates significant protective effects against abdominal sepsis in mice.
  • The therapeutic potential of DPAS is attributed to enhanced bacterial clearance and modulation of inflammatory responses.
  • DPAS represents a promising novel therapeutic strategy for abdominal sepsis.

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