Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induction01:16

Induction

5.7K
An emf is induced when the magnetic field in a coil is changed by pushing a bar magnet into or out of the coil. emfs of opposite signs are produced by motion in opposite directions, and the directions of emfs are also reversed by reversing poles. The same results are produced if the coil is moved rather than the magnet—it is the relative motion that is important. The faster the motion, the greater the emf. Additionally, there is no emf when the magnet is stationary relative to the coil.
A...
5.7K
Self-Inductance01:24

Self-Inductance

3.1K
Mutual inductance arises when a current in one circuit produces a changing magnetic field that induces an emf in another circuit. On the other hand, self-inductance arises when the current passing through the circuit changes, creating a changing magnetic flux, resulting in inductance in the same circuit.
Consider a circuit connected to an AC source. As the current varies with time, the magnetic flux through the circuit correspondingly changes. Faraday's law tells us that an emf would...
3.1K
Mutual Inductance01:24

Mutual Inductance

3.8K
Inductance is the property of a device that tells us how effectively it induces an emf in another device. In other words, it is a physical quantity that expresses the effectiveness of a given device.
When two circuits carrying time-varying currents are close to one another, the magnetic flux through each circuit varies because of the changing current in the other circuit. Consequently, an emf is induced in each circuit by the changing current in the other. Therefore, this type of emf is called...
3.8K
Inductive Reasoning00:59

Inductive Reasoning

67.8K
Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. It is uncertain and operates in degrees to which the conclusions are credible. As such, inductive arguments can be weak or strong, rather than valid or invalid, and conclusions can be used to formulate testable, falsifiable hypotheses.
Inductive reasoning is common in descriptive science. A life scientist makes observations and records them. This data can be qualitative or...
67.8K
Mathematical Induction01:29

Mathematical Induction

279
Mathematical induction is a structured method of proof used to confirm the truth of statements involving natural numbers. Consider the sum of the first n natural numbers:This formula describes a pattern that appears to hold true as more terms are added. To verify that it is valid for all natural numbers, mathematical induction proceeds in two essential steps. The first is the base case, where the formula is tested for the initial value, typically n = 1. Substituting into both sides confirms the...
279
Calculation of Self-inductance01:29

Calculation of Self-inductance

846
The self-inductance of a circuit, often simply called the inductance, is a purely geometric factor that depends only on the circuit component's structure. More specifically, it depends on the shape and size of the component that lets the flux pass through it, thus inducing an electric field that opposes any current passing through it.
Since the effect of the induced electric field and the back EMF generated depends on the rate of change of current and the self-inductance, the inductance...
846

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systemic Lupus Erythematosus and Epstein-Barr Virus: Recent Discoveries and Open Questions.

Clinical chemistry·2026
Same author

Concurrent Anti-PR3 Immunoassay and cANCA Indirect Immunofluorescence Testing Provide Complementary Information for Clinical Laboratory Detection of Antineutrophil Cytoplasmic Antibodies.

The journal of applied laboratory medicine·2025
Same author

Tafasitamab interference in immunofixation electrophoresis.

Clinical chemistry and laboratory medicine·2025
Same author

The Therapeutic Monoclonal Antibody Pemivibart Causes Persistent Interference in Immunofixation Electrophoresis.

The journal of applied laboratory medicine·2025
Same author

Reference errors in microbiology literature: 'pyroptosis' and others.

Trends in microbiology·2024
Same author

Broad-spectrum inflammasome inhibition by thiomuscimol.

Cell death discovery·2024
Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020
Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020
Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020
See all related articles

Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

2.2K

Pyroptosis Induction and Detection.

Andreas B den Hartigh1, Susan L Fink1

  • 1Department of Laboratory Medicine, University of Washington, Seattle, Washington.

Current Protocols in Immunology
|July 21, 2018
PubMed
Summary
This summary is machine-generated.

Pyroptosis, a programmed inflammatory cell death, is triggered by caspase-1 activation. This process involves gasdermin D cleavage, leading to cell lysis and cytokine release, crucial for host defense and inflammation.

Keywords:
caspase-1cell deathinflammasomepyroptosis

More Related Videos

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells
07:47

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Published on: January 21, 2020

7.5K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K

Related Experiment Videos

Last Updated: Feb 7, 2026

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

2.2K
Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells
07:47

Apoptosis Induction and Detection in a Primary Culture of Sea Cucumber Intestinal Cells

Published on: January 21, 2020

7.5K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Pyroptosis is a programmed inflammatory cell death pathway.
  • It is mediated by caspase-1, a cysteine protease.
  • Pyroptosis plays a dual role in host defense and pathogenic inflammation.

Purpose of the Study:

  • To describe methods for stimulating pyroptosis.
  • To outline techniques for assessing plasma membrane integrity loss during pyroptosis.
  • To present an ELISA for quantifying released interleukin-1β (IL-1β).

Main Methods:

  • Stimulation of pyroptosis in cellular models.
  • Assessment of plasma membrane integrity.
  • Enzyme-linked immunosorbent assay (ELISA) for IL-1β quantification.

Main Results:

  • Established protocols for inducing and measuring pyroptosis.
  • Quantified IL-1β release using ELISA.
  • Demonstrated the utility of these methods across various experimental contexts.

Conclusions:

  • The described methods provide a framework for studying pyroptosis.
  • These techniques facilitate the investigation of pyroptosis's role in infection and inflammation.
  • The methods are versatile and applicable to diverse research applications.