Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.3K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.3K
Membrane Lipids01:32

Membrane Lipids

34.3K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
34.3K
Membrane Proteins01:30

Membrane Proteins

30.5K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.5K
Structural Protein Function01:56

Structural Protein Function

30.0K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity.  In bones and teeth, it mineralizes to...
30.0K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.7K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
5.7K
What are Lipids?01:38

What are Lipids?

220.7K
Overview
220.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bisphosphonate zoledronic acid blocks secretory autophagy and inhibits bone resorptive functions in osteoclasts.

Autophagy·2026
Same author

Drug Development.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

T cell cholesterol transport links intestinal immune responses to dietary lipid absorption.

Science (New York, N.Y.)·2025
Same author

Inhalation of electrophilic and redox active electronic cigarette aerosol increases oxidative potential in the lung in an acute manner.

Free radical biology & medicine·2025
Same author

In situ insights into antibody-mediated neutralization of a pre-fusion Junin virus glycoprotein complex.

Cell reports·2025
Same author

Proinflammatory macrophages release CXCL5 to regulate T cell function and limit effects of αPD-1 in steatosis-driven liver cancer.

JHEP reports : innovation in hepatology·2025

Related Experiment Video

Updated: Feb 7, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.6K

Lipid Modulation of Membrane Protein Function.

Julian Whitelegge1

  • 1The Pasarow Mass Spectrometry Laboratory, David Geffen School of Medicine, University of California Los Angeles, NPI-Semel Institute, 760 Westwood Plaza, Los Angeles, CA 90095, USA.

Cell Chemical Biology
|July 21, 2018
PubMed
Summary

Native mass spectrometry reveals phosphatidyl-inositol

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Integral membrane proteins play crucial roles in cellular processes.
  • Lipid-protein interactions are vital for membrane protein function and regulation.
  • Native mass spectrometry is a key technique for studying these interactions.

Purpose of the Study:

  • To investigate the role of phosphatidyl-inositol in the function of the eukaryotic purine transporter, UapA.
  • To elucidate the mechanisms of UapA dimerization and activity.

Main Methods:

  • Native mass spectrometry was employed to study UapA.
  • Analysis of lipid-protein complexes involving phosphatidyl-inositol and UapA.

Main Results:

More Related Videos

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
20:21

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: September 1, 2012

18.1K
Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

31.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

30.6K
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
20:21

Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: September 1, 2012

18.1K
Crystallization of Membrane Proteins in Lipidic Mesophases
11:53

Crystallization of Membrane Proteins in Lipidic Mesophases

Published on: March 28, 2011

31.8K
  • Phosphatidyl-inositol was identified as a key molecule interacting with UapA.
  • This interaction is critical for the dimerization of UapA.
  • Phosphatidyl-inositol binding influences the activity of UapA.

Conclusions:

  • Phosphatidyl-inositol plays a significant role in regulating the function of the eukaryotic purine transporter UapA.
  • UapA dimerization and activity are modulated by phosphatidyl-inositol binding.
  • Native mass spectrometry provides valuable insights into lipid-protein interactions in membrane transport.