The interactions of fullerene C₆₀ and Benzo(α)pyrene influence their bioavailability and toxicity to zebrafish...

This study aimed to assess the toxicological consequences related to the interaction of fullerene nanoparticles (C₆₀) and Benzo(α)pyrene (B(α)P) on zebrafish embryos, which were exposed to C₆₀ and B(α)P alone and to C₆₀ doped with B(α)P. The uptake of pollutants into their tissues and intra-cellular localization were investigated by immunofluorescence and electron microscopy. A set of biomarkers of genotoxicity and oxidative stress, as well as functional proteomics analysis were applied to assess the toxic effects due to C₆₀ interaction with B(α)P. The carrier role of C₆₀ for B(α)P was observed, however adsorption on C₆₀ did not affect the accumulation and localization of B(α)P in the embryos. Instead, C₆₀ doped with B(α)P resulted more prone to sedimentation and less bioavailable for the embryos compared to C₆₀ alone. As for toxicity, our results suggested that C₆₀ alone elicited oxidative stress in embryos and a down-regulation of proteins involved in energetic metabolism. The C₆₀ + B(α)P induced cellular response mechanisms similar to B(α)P alone, but generating greater cellular damages in the exposed embryos.