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Related Concept Videos

Aggregates Classification01:29

Aggregates Classification

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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
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RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Bonding and Strength of Aggregate01:12

Bonding and Strength of Aggregate

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The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
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Specific Gravity of Aggregate01:19

Specific Gravity of Aggregate

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Aggregates typically contain pores, which can be either permeable or impermeable. Considering the pores in the aggregates, the specific gravity of aggregates is defined in three different forms, namely, bulk or gross specific gravity, apparent specific gravity, and absolute specific gravity.
Bulk or gross specific gravity is calculated by taking the ratio of the mass of aggregates in the saturated surface-dry state to the total volume that includes both the solids and the voids within the...
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Bulk Density of Aggregate01:22

Bulk Density of Aggregate

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Bulk density refers to the mass of aggregate particles that would fill a unit volume. The concept of bulk density originates from the inability to pack aggregate particles in a manner that completely eliminates void spaces. Hence, the term bulk refers to the volume that encompasses both the aggregates and the voids. This measurement is crucial when aggregates are batched by volume and is used to convert quantities by mass to volume.
Most natural mineral aggregates, like sand and gravel,...
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Toughness and Hardness of Aggregate01:22

Toughness and Hardness of Aggregate

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Toughness and hardness are critical properties of aggregate materials used in concrete, particularly on pavement surfaces and industrial flooring subjected to heavy loads. Toughness is defined as the aggregate's resistance to failure by impact and is measured by the aggregate impact value (AIV). For this, the aggregate impact value test is performed, wherein the impact is delivered by a standard hammer, which falls freely under its own weight onto the aggregates. The aggregates fragment in...
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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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Multivalent aptasensor array and silver aggregated amplification for multiplex detection in microfluidic devices.

Xiaohui Liu1, Hui Li1, Yaju Zhao1

  • 1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, China.

Talanta
|July 22, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic aptasensor for detecting protein biomarkers. The multivalent aptasensor array and silver aggregation strategy enable rapid and sensitive detection of PDGF-BB and VEGF-165.

Keywords:
AptamerMicroarrayMicrofluidicMultiplex detectionSilver nanoparticles

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Area of Science:

  • Biomarker detection
  • Microfluidics
  • Nanotechnology

Background:

  • Accurate and sensitive detection of protein biomarkers is crucial for early disease diagnosis.
  • Existing methods often face challenges in speed, sensitivity, and multiplexing capabilities.

Purpose of the Study:

  • To develop a rapid, sensitive, and multiplexed aptamer-based assay for protein biomarker detection.
  • To integrate a multivalent aptasensor array (MAA) chip with a silver aggregation amplification (SAA) strategy within a microfluidic device.

Main Methods:

  • Fabrication of an MAA chip by immobilizing aptamer-modified silver nanoparticles.
  • Construction of a microfluidic device using polydimethylsiloxane (PDMS).
  • Utilizing aptamer-protein interactions and silver nanoparticle aggregation for signal amplification and fluorescence detection.

Main Results:

  • Achieved a linear response for PDGF-BB detection from 16 pg/mL to 250 ng/mL with a detection limit of 1.4 pg/mL.
  • Demonstrated sensitive PDGF-BB detection in 10% blood serum (limit of detection 7.8 pg/mL).
  • Successfully performed simultaneous detection of PDGF-BB and VEGF-165 with good specificity and sensitivity.

Conclusions:

  • The developed microfluidic aptasensor offers a rapid and sensitive platform for biomarker detection.
  • The MAA chip and SAA strategy provide a robust method for signal amplification.
  • This assay platform holds potential for high-throughput screening of multiple protein biomarkers.