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Updated: Feb 7, 2026

Fabrication of Amperometric Electrodes
Published on: May 4, 2009
Amperometric immunosensor for prolactin hormone measurement using antibodies loaded on a nano-Au monolayer modified
Hadi Beitollahi1, Samira Nekooei2, Masoud Torkzadeh-Mahani3
1Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Abstract:
The fabrication of a novel electrochemical immunosensor for rapid and precise determination of prolactin was carried out using carbon paste electrode (CPE) consist of ionic liquid (IL) and graphite. Gold nanoparticles were employed as a modifier on the surface of CPE to immobilize the prolactin antibody (anti-PRL). The immunoassay was set up by sandwiching the antigen between prolactin antibody and the polyclonal anti-human-prolactin antibody labeled with horseradish peroxidase (HRP-labeled anti-PRL) as secondary antibody, on the surface of modified CPE. The reaction between O-aminophenol (OAP) and H2O2 which is catalyzed by labeled HRP on the sandwich immunosensor generate a signal in differential pulse voltammetry (DPV) that is used to determine the concentration of prolactin. This immunosensor provides the measurement of prolactin concentration in a linear range of 25.0-2000.0 mIU L-1 with a detection limit 12.5 mIU L-1. Moreover, it is applicable in the clinical assay of prolactin due to its high sensitivity and acceptable stability.
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