Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Routes of Persuasion

Routes of Persuasion

Persuasion is the process of changing our attitude toward something based on some kind of communication. Much of the persuasion we experience comes from outside forces. How do people convince others to change their attitudes, beliefs, and behaviors? What communications do you receive that attempt to persuade you to change your attitudes, beliefs, and behaviors?

Molecular Shapes

Molecular Shapes

Molecules have characteristic shapes that are crucial for their function. The arrangement of various electron groups around the central atom dictates their molecular geometry. Electron pairs in the valence shell of a central atom will adopt an arrangement that minimizes repulsions between the electron pairs by maximizing the distance between them. The valence electrons form either bonding pairs, located primarily between bonded atoms, or lone pairs.
Two regions of electron density in a diatomic...

Protein Organization

Protein Organization

Protein Folding

Protein Folding

Conserved Binding Sites

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Modular microfluidic probe for addressable fluidic landscapes.

Lab on a chip·2026

Same author

Multichannel soft microfluidic force sensors: design, characterization, and application in laparoscopy.

Microsystems & nanoengineering·2026

Same author

Use of microfluidic "chip" devices for assessing the effect of radiation on 3D cell culture models.

Methods in cell biology·2026

Same author

ENN A1 and B1 In Vitro Toxicological Effects on 2D and 3D Organ-on-Chip HepaRG Liver Cells.

Toxins·2026

Same author

Twenty years of microfluidic probes and open-space microfluidics: from origins to emerging directions.

Lab on a chip·2026

Same author

Spheromatrix: a paper-based platform for scalable 3D tumor model generation, cryopreservation, and high-throughput drug assessment.

Microsystems & nanoengineering·2025

Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026

Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026

Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026

Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026

Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026

Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 7, 2026

3D Printing - Evaluating Particle Emissions of a 3D Printing Pen

3D Printing - Evaluating Particle Emissions of a 3D Printing Pen

Published on: October 9, 2020

3D Printed Microfluidic Probes.

Ayoola Brimmo^1,2, Pierre-Alexandre Goyette³, Roaa Alnemari¹

¹Division of Engineering, New York University Abu Dhabi, Abu Dhabi, UAE.

Scientific Reports

|July 22, 2018

Summary

This summary is machine-generated.

Stereolithographic 3D printing enables rapid, inexpensive fabrication of microfluidic probes (MFPs). These 3D printed MFPs demonstrate performance comparable to traditional methods, democratizing microfluidic technology for broader research applications.

More Related Videos

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

3D Printing of Biomolecular Models for Research and Pedagogy

3D Printing of Biomolecular Models for Research and Pedagogy

Published on: March 13, 2017

Related Experiment Videos

Last Updated: Feb 7, 2026

3D Printing - Evaluating Particle Emissions of a 3D Printing Pen

3D Printing - Evaluating Particle Emissions of a 3D Printing Pen

Published on: October 9, 2020

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Generation of Tissue Spheroids via a 3D Printed Stamp-Like Device

Published on: October 6, 2022

3D Printing of Biomolecular Models for Research and Pedagogy

3D Printing of Biomolecular Models for Research and Pedagogy

Published on: March 13, 2017

Area of Science:

Microfluidics
3D Printing
Biotechnology

Background:

Microfluidic probes (MFPs) are crucial for precise reagent delivery and gradient generation.
Traditional MFP fabrication relies on complex and expensive glass or silicon micromachining.
There is a need for more accessible and customizable MFP fabrication methods.

Purpose of the Study:

To develop and validate a single-step fabrication protocol for MFPs using stereolithographic 3D printing.
To benchmark the performance of 3D printed MFPs against conventionally fabricated MFPs.
To assess the impact of 3D printing resolution on MFP functionality.

Main Methods:

Fabrication of MFPs using stereolithographic 3D printing with two independent designs and protocols.
Flow characterization by reproducing microfluidic dipolar and quadrupolar reagent delivery profiles.
Comparison of experimental results with numerical simulations and scaling laws.

Main Results:

3D printed MFPs were successfully fabricated in a single step using inexpensive stereolithography printers.
Performance of 3D printed MFPs closely matched theoretical predictions and conventional methods for most applications.
Printer resolution artifacts had negligible impact on probe operation, reagent patterning, and cell staining.

Conclusions:

Stereolithographic 3D printing offers a simple, inexpensive, and highly customizable method for MFP fabrication.
3D printed MFPs are suitable for cell processing and generating microfluidic concentration gradients, even with moderate resolution printers.
This 3D printing approach has the potential to democratize microfluidic technology for a wider range of research groups.