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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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The study of music provides many examples of the superposition of waves and the constructive and destructive interference that occurs. Very few examples of music being performed consist of a single source playing a single frequency for an extended period of time. A single frequency of sound for an extended period might be monotonous to the point of irritation, similar to the unwanted drone of an aircraft engine or a loud fan. Music is pleasant and exciting due to mixing the changing frequencies...
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Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
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Accelerators in concrete serve as admixtures to speed up the hardening process, enabling the concrete to achieve early strength faster. Although accelerators do not necessarily impact the time it takes concrete to set, they reduce this time in practice. A common accelerator is calcium chloride, which is particularly useful for hastening early strength development in cold weather or for rapid repair jobs that require quick heat generation after mixing.
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Interference and Decay01:16

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RNA Interference in Ticks
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To accelerate the Zika beat: Candidate design for RNA interference-based therapy.

M Giulietti1, A Righetti1, L Cianfruglia1

  • 1Department of Specialistic Clinical and Odontostomatological Sciences, Polytechnic University of Marche, Ancona, 60131, Italy.

Virus Research
|July 22, 2018
PubMed
Summary
This summary is machine-generated.

Bioinformatic analysis identified promising short interfering RNAs (siRNAs) to combat Zika virus replication. These siRNAs target conserved regions and show potential broad-spectrum antiviral activity and safety for therapeutic development.

Keywords:
Genome alignmentOff-TargetRNA interferenceSafetyZikasiRNA design

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Area of Science:

  • Virology
  • Bioinformatics
  • RNA Therapeutics

Background:

  • Zika virus causes severe neurological disorders, with no current effective therapy.
  • Short interfering RNAs (siRNAs) are a potential treatment, showing success against other viruses.

Purpose of the Study:

  • To computationally design effective and safe siRNAs against Zika virus.
  • To identify siRNAs with broad-spectrum antiviral activity and minimal off-target effects.

Main Methods:

  • Comprehensive bioinformatic analysis of Zika virus genomes.
  • In silico screening of siRNAs for efficacy, conserved targeting, and safety (immune response, host RNA/protein interactions).

Main Results:

  • Identified highly conserved, targetable regions in the Zika genome.
  • Selected a limited number of siRNAs meeting stringent safety criteria.
  • Two candidate siRNAs demonstrated efficacy against other flaviviruses in vitro and in vivo.

Conclusions:

  • The study presents a short list of promising siRNA candidates for Zika virus treatment.
  • These siRNAs target conserved flavivirus regions, suggesting potential broad efficacy.
  • Experimental validation of these computationally designed siRNAs is encouraged.