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Development and evaluation of a spray applicator for platelet-rich plasma
Katharina Düregger1, Alexander Gäble1, Markus Eblenkamp1
1Institute of Medical and Polymer Engineering, Department of Mechanical Engineering, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, Germany.
Colloids and Surfaces. B, Biointerfaces
|July 23, 2018
Summary
A new compact spray device effectively applies platelet-rich blood derivatives for wound healing. This technology ensures even distribution and minimal cell damage, offering a promising tool for medical applications.
Area of Science:
- Biomedical Engineering
- Regenerative Medicine
- Wound Healing Technologies
Background:
- Platelet-rich blood derivatives are valuable for wound healing due to released growth factors.
- Efficient application of these derivatives to large wound surfaces is challenging.
- Spraying offers a method for uniform delivery of blood derivatives.
Purpose of the Study:
- To develop a compact, self-contained spraying device for autologous platelet-rich blood derivatives.
- To evaluate the device's spray characteristics and its impact on cellular components.
- To assess the biological efficacy of the sprayed derivatives for wound healing applications.
Main Methods:
- A novel 3D-printed, two-substance nozzle was designed for aspiration and atomization using a biocompatible propellant (R134a).
- Spray characteristics were analyzed through flow volume, cone angle, and droplet size measurements.
- Biological evaluations included flow cytometry for platelet activation, PDGF ELISA for growth factor release, and scanning electron microscopy for cell morphology.
Main Results:
- The developed spraying device demonstrated excellent spray characteristics with minimal mechanical stress on blood cells.
- Platelet activation and growth factor release were preserved post-spraying.
- Sprayed blood derivatives maintained homogeneity and cellular morphology.
Conclusions:
- The compact, self-contained spraying device is a viable tool for applying platelet-rich blood derivatives.
- The device ensures even distribution and preserves the biological integrity of the therapeutic components.
- This technology holds significant promise for enhancing wound healing treatments.
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