Proteomics analysis of Xiangcaoliusuobingmi-treated Capsicum annuum L. infected with Cucumber mosaic virus
Jing Shi1, Lu Yu1, Baoan Song1
1State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, PR China.
Abstract:
Among different viruses, Cucumber mosaic virus (CMV) has the most extensive host range, being capable of infecting over 1200 species, and causes severe damage worldwide. Xiangcaoliusuobingmi (B1), a candidate plant immune activator drug, exhibited significant protective effects against CMV. However, its potential mechanism is still unknown. In this study, we found the defensive enzyme activities of peroxidase (POD), phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD) and catalase (CAT) can be enhanced by B1. Meanwhile, we found RT-qPCR assay results of the defensive gene expression can be improved by B1 in capsicum. Moreover, we analyze the result of label-free proteomics, B1 could trigger abscisic acid (ABA) pathway. All data provide a more understanding about the response to infect CMV capsicum activeted by B1 in the level of the plant physiology and biochemistry, gene and protein.
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