Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Structures of Solids02:22

Structures of Solids

17.9K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
17.9K
Phase Diagrams02:39

Phase Diagrams

50.3K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
50.3K
Phase Transitions02:31

Phase Transitions

23.2K
Whether solid, liquid, or gas, a substance's state depends on the order and arrangement of its particles (atoms, molecules, or ions). Particles in the solid pack closely together, generally in a pattern. The particles vibrate about their fixed positions but do not move or squeeze past their neighbors. In liquids, although the particles are closely spaced, they are randomly arranged. The position of the particles are not fixed—that is, they are free to move past their neighbors to...
23.2K
Metallic Solids02:37

Metallic Solids

20.8K
Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties.
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
20.8K
Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

6.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.5K
The Unfolded Protein Response01:37

The Unfolded Protein Response

6.4K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Hydrophilic Brush Length and Hydrophobic Chain on Biodistribution of Polymethacrylate-Based Statistical Copolymers.

Biomacromolecules·2026
Same author

Physiotherapists' pro-growth experiences in past and current workplaces: a cross-sectional study.

Journal of physical therapy science·2026
Same author

Replicative and stress-induced premature senescence distinctively affect the endothelial anticoagulation capacity.

PloS one·2026
Same author

Hepatocyte purification column using thermoresponsive glycopolymer-modified silica beads.

Journal of materials chemistry. B·2026
Same author

Model-Based Financial Consequences of Electrical Stimulation Therapy for Pressure Injury Healing.

Healthcare (Basel, Switzerland)·2026
Same author

Interface pressure reduction in the sacral and ischial regions during neuromuscular electrical stimulation at 60° head-of-bed elevation.

Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine·2026

Related Experiment Video

Updated: Feb 7, 2026

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

5.2K

Protein purification using solid-phase extraction on temperature-responsive hydrogel-modified silica beads.

Kohei Okubo1, Koji Ikeda1, Ayaka Oaku1

  • 1Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan.

Journal of Chromatography. A
|July 24, 2018
PubMed
Summary

A novel temperature-responsive solid-phase extraction (SPE) column effectively purifies biopharmaceuticals. This method preserves protein bioactivity, crucial for therapeutic applications.

Keywords:
Monoclonal antibodyPoly(N-isopropylacrylamide)Protein activityProtein purificationRituximabTemperature-responsive polymer

More Related Videos

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

12.4K
Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.2K

Related Experiment Videos

Last Updated: Feb 7, 2026

Ganglioside Extraction, Purification and Profiling
10:05

Ganglioside Extraction, Purification and Profiling

Published on: March 12, 2021

5.2K
A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

12.4K
Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification
08:51

Preparation of Polypentafluorophenyl acrylate Functionalized SiO2 Beads for Protein Purification

Published on: November 19, 2018

10.2K

Area of Science:

  • Biochemistry
  • Materials Science
  • Chemical Engineering

Background:

  • Biopharmaceuticals are increasingly vital in medicine, necessitating efficient purification techniques.
  • Maintaining protein bioactivity during purification is critical for therapeutic efficacy.
  • Existing purification methods can sometimes compromise protein integrity.

Purpose of the Study:

  • To develop a temperature-responsive solid-phase extraction (SPE) column for protein purification.
  • To ensure that the purification process does not affect the bioactivity of the proteins.
  • To create a novel method for purifying biopharmaceuticals.

Main Methods:

  • Coating aminopropyl silica beads with poly(N-isopropylacrylamide) (PNIPAAm)-based thermoresponsive hydrogels.
  • Copolymerizing PNIPAAm with hydrophobic (n-butyl methacrylate) and anionic (acrylic acid) monomers.
  • Utilizing temperature changes (40°C to 4°C) for protein retention and elution.

Main Results:

  • Successful separation of lysozyme from ovalbumin with 99.7% bioactivity retention.
  • Purification of Rituximab (monoclonal antibody) from BSA and hybridoma cell culture medium.
  • No denaturation of Rituximab observed in the eluted fraction.

Conclusions:

  • Temperature-responsive polymer-based SPE is a viable method for biomedical purifications.
  • This technique effectively purifies proteins while preserving their biological activity.
  • The developed SPE column offers a promising approach for biopharmaceutical production.