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Updated: Feb 7, 2026

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Published on: August 17, 2022
Serial integration of Dean-structured sample cores with linear inertial focussing for enhanced particle and cell
Paul M Holloway1, Jonathan Butement1, Manjunath Hegde
1Centre for Hybrid Biodevices and Cancer Sciences, Faculty of Medicine, University of Southampton, Southampton SO17 1BJ, United Kingdom.
Abstract:
In this contribution, a channel aspect ratio of >2 was used to access high velocity regimes to provide confined sample cores by Dean focussing in advance of linear inertial focussing. This produces a singular separation origin with a mirrored transport path for efficient particle and blood cell sorting, while also increasing the spatial resolution for multiscale sorting.
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