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Tn7-encoded proteins.

J Brevet, F Faure, D Borowski

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified six proteins encoded by the Tn7 transposon in Escherichia coli. Four specific proteins (p85-a, p54, p40, p85-b) are essential for Tn7 transposition onto plasmids, but not all are needed for chromosomal transposition.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • The Tn7 transposon is a mobile genetic element with significant implications in bacterial genetics.
    • Understanding the proteins encoded by Tn7 is crucial for elucidating its transposition mechanisms.

    Purpose of the Study:

    • To identify and characterize proteins encoded by the Tn7 transposon in Escherichia coli.
    • To determine the roles of these proteins in Tn7 transposition.

    Main Methods:

    • Analysis of proteins expressed by Tn7 in Escherichia coli maxicells using various ColE1::Tn7 plasmids and pBR322 clones.
    • Detection and molecular weight determination of Tn7-encoded proteins.

    Main Results:

    • Six Tn7-encoded proteins were identified: p18, p32, p40, p54, p85-a, and p85-b.

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  • Protein p18 is dihydrofolate reductase type I; p32 likely confers streptomycin/spectinomycin resistance.
  • Genes for p18 and p32 are on the left of Tn7; genes for p85-a, p54, p40, and p85-b are on the right, arranged contiguously.
  • Transposition to plasmids requires p85-a, p85-b, p54, and p40; transposition to the chromosome requires only p85-a and p54.

    • Conclusions:

    • The study delineates the protein-coding regions of Tn7 and their functional involvement in transposition.
    • Differential requirements for transposition proteins suggest distinct mechanisms for plasmid versus chromosomal integration.