Related Experiment Video

Updated: Feb 7, 2026

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
07:28

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

3.6K

FGF2 Antiproliferative Stimulation Induces Proteomic Dynamic Changes and High Expression of FOSB and JUNB in

Francisca Nathalia de Luna Vitorino1, Fabio Montoni1, Jaqueline Neves Moreno1

  • 1Laboratório Especial de Ciclo Celular - Center of Toxins, Immune-Response and Cell Signaling - CeTICS, Instituto Butantan, São Paulo, SP, 05503-900, Brazil.

Proteomics
|July 24, 2018
PubMed

Insights

Fibroblast growth factor 2 (FGF2) can halt cell proliferation by altering cellular processes. This study reveals FGF2 impacts metabolism and DNA replication via transcription factors FOSB and JUNB, with FOSB delaying S-phase progression.

Area of Science:

  • Molecular Biology
  • Proteomics
  • Cancer Research

Background:

  • Fibroblast growth factor 2 (FGF2) is known to promote cell proliferation but can also induce cell cycle arrest.
  • The molecular mechanisms underlying FGF2's antiproliferative effects remain incompletely understood.
  • Investigating early proteomic changes offers insight into FGF2-induced growth inhibition.

Purpose of the Study:

  • To elucidate the early systemic proteomic differences induced by FGF2 in a K-Ras-driven mouse tumor cell line.
  • To identify key molecular players and pathways regulated by FGF2 during cell cycle arrest.
  • To investigate the role of transcription factors FOSB and JUNB in FGF2's antiproliferative effects.

Main Methods:

  • Quantitative proteomics approach to analyze protein expression changes.
  • Analysis of over 2900 proteins in a K-Ras-driven mouse tumor cell line (Y1) upon FGF2 stimulation.
  • Transcription regulatory network analysis and gene knockdown experiments in Y1, SK-N-MC, and UM-UC-3 cell lines.

Main Results:

  • FGF2 stimulation enriched proteins associated with metabolism, RNA processing, replication, and transcription.
  • FGF2 delayed abundance changes for proteins involved in DNA replication and carbohydrate metabolism.
  • FOSB and JUNB were identified as master regulators; FOSB knockdown rescued S-phase progression delay but not overall growth arrest.

Conclusions:

  • FGF2 induces complex proteomic changes impacting metabolism and cell cycle regulation.
  • FOSB plays a critical role in mediating the S-phase progression delay induced by FGF2.
  • Understanding these FGF2-driven pathways provides insights into potential therapeutic strategies for FGF2-responsive cancers.
Keywords:
DNA replicationFGF2FOSBJUNBantiproliferativeproteomicssrc

More Related Videos

Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria08:11

Measuring Growth and Gene Expression Dynamics of Tumor-Targeted S. Typhimurium Bacteria

Published on: July 6, 2013

13.3K
The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics10:31

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Published on: October 6, 2016

11.9K

Related Concept Videos

The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
7.3K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
5.5K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K