Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

17.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.3K
Mate Choice01:20

Mate Choice

11.8K
Mate choice—the decision about whom to mate with—is a type of natural selection, since animals must reproduce to pass down their genes. Mate choice is also called intersexual selection because the behavior occurs between the sexes.
11.8K
Natural Selection and Mating Preferences01:06

Natural Selection and Mating Preferences

550
The principle of natural selection posits that organisms better adapted to their environment are more likely to survive and reproduce. This principle is closely intertwined with mating preferences, a key aspect of sexual selection, which evolutionary psychologists believe is driven by instincts to propagate one's genes. Such instincts significantly influence mating behaviors and preferences between genders.
Females, due to their biological roles in conception, pregnancy, and nursing,...
550
Genetic Screens02:46

Genetic Screens

5.7K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.7K
Lewis Acids and Bases02:33

Lewis Acids and Bases

48.4K
In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
48.4K
Base Excision Repair01:54

Base Excision Repair

26.4K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging mass cytometry reveals novel cellular neighborhoods for predicting the prognosis of nonalcoholic fatty liver disease-associated hepatocellular carcinoma.

Cancer cell international·2026
Same author

Efficacy and safety of <sup>99m</sup>Tc-MIBI SPECT-guided botulinum toxin injection in parkinsonian postural abnormality.

BMC neurology·2026
Same author

RNA-Binding Protein TAF15 Suppresses Toxicity in a Yeast Model of FUS Proteinopathy.

Journal of fungi (Basel, Switzerland)·2026
Same author

Exercise-Conditioned Endothelial Progenitor Cell-Exosomes Preserve Cerebral Blood Flow and Alleviate Acute Ischemic Brain Injury in Hypertensive Mice.

Life (Basel, Switzerland)·2026
Same author

Experimental assessment of AI-based interactome mapping.

Nature communications·2026
Same author

Polydopamine-assisted lithium ion loading on titanium alloy synergistically enhances osteogenesis and immunomodulation for bone regeneration: an In Vitro study.

BMC oral health·2026

Related Experiment Video

Updated: Feb 7, 2026

Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K

Mating-based Overexpression Library Screening in Yeast.

Elliott Hayden1, Shuzhen Chen1, Abagail Chumley1

  • 1Department of Biological Sciences, Wright State University.

Journal of Visualized Experiments : Jove
|July 24, 2018
PubMed
Summary

This study introduces a new yeast screening method using mating to efficiently identify modifiers of toxic proteins, like Amyotrophic Lateral Sclerosis-associated FUS (Fused in Sarcoma). This platform simplifies genetic screening for disease-associated protein toxicity.

More Related Videos

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.8K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

8.2K
High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.8K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K

Area of Science:

  • Biochemistry
  • Genetics
  • Neuroscience

Background:

  • Budding yeast is a model organism for studying human disease proteins.
  • Genome-wide genetic screening is crucial for yeast-based disease research.
  • Neurodegenerative disease proteins in yeast can cause cytotoxicity and aggregation, mirroring human conditions.

Purpose of the Study:

  • To develop and validate a novel screening method for identifying genetic modifiers of toxicity.
  • To screen for modifiers of the Amyotrophic Lateral Sclerosis-associated protein Fused in Sarcoma (FUS) in a yeast model.

Main Methods:

  • A new yeast screening platform utilizing yeast mating to introduce arrayed plasmid libraries.
  • Storing pre-transformed plasmid libraries as glycerol stocks for long-term use.
  • Applying the mating method to bypass labor-intensive yeast transformations for screening.

Main Results:

  • Demonstrated the successful application of the mating-based screening method.
  • Identified genes that modify the toxicity of the FUS protein in the yeast model.

Conclusions:

  • The yeast mating method is a highly efficient and robust platform for genetic screening.
  • This method facilitates the discovery of novel genetic modifiers for disease-associated proteins like FUS.
  • The platform offers a labor-saving and storable approach for future yeast-based genetic screens.