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Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
Published on: April 15, 2010
Maximizing the sensitivity in 13C cross-polarization magic-angle-spinning solid-state NMR measurements with flip-back
Yue-Qi Ye1, Charlotte Martineau-Corcos2, Francis Taulelle3
1JEOL Beijing Co. Ltd., Haidian District, Beijing 100190, China; JEOL RESONANCE Inc., Musashino, Akishima, Tokyo 196-8558, Japan.
Abstract:
The flip-back pulse combined with cross-polarization magic-angle spinning (CPMAS-FB) has been widely used to reduce the optimal repetition delay, resulting in enhancing sensitivity per unit time. Despite its common use in samples with long 1H T1 relaxation time, the experimental setup of the repetition delay in CPMAS-FB is not obvious. In this article, a simple model is used to derive the optimal repetition delay and expected sensitivity gain through the 1H T1 relaxation time and signal decay during 1H spinlock.
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