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Updated: Feb 7, 2026
Author Spotlight: Optimizing Bovine Lung Decellularization for Organotypic Hydrogels
Published on: December 8, 2023
Impact of different intratracheal flows during lung decellularization on extracellular matrix composition and
Mariana Alves Bellezzia1,2, Fernanda F Cruz1,2, Vanessa Martins1,3
1Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, RJ, Brazil.
Aim:
To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established lung decellularization protocol.
Materials & Methods:
Healthy mice were used: 15 for decellularization and five to serve as controls. Fluids were instilled at 5, 10 and 20 ml/min flow rates through tracheal cannula and right ventricular cavity (0.5 ml/min) in all groups.
Results:
The 20 ml/min rate better preserved collagen content in decellularized lungs. Elastic fiber content decreased at 5 and 10 ml/min, but not at 20 ml/min, compared with controls. Chondroitin, heparan and dermatan content was reduced after decellularization.
Conclusion:
An intratracheal flow rate of 20 ml/min was associated with lower resistance and greater preservation of collagen to that observed in ex vivo control lungs.
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