Fluorescent probes for detecting cysteine
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Updated: Feb 7, 2026

Synthesis of Protein Bioconjugates via Cysteine-maleimide Chemistry
Published on: July 20, 2016
Fanyong Yan1, Xiaodong Sun1, Fanlin Zu1
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Key Lab of Fiber Modification & Functional Fiber of Tianjin, Tianjin Polytechnic University, Tianjin 300387, People's Republic of China.
Abstract:
Cysteine plays a crucial role in physiological processes. Therefore, it is necessary to develop a method for detecting cysteine. Fluorimetry has the advantages of convenient detection, short response time, high sensitivity and good selectivity. In this review, fluorescent probes that detect cysteine over the past three years are summarized based on structural features of fluorophores such as coumarin, BODIPY, rhodamine, fluorescein, CDs, QDs, etc and reaction groups including acrylate, aldehyde, halogen, 7-nitrobenzofurazan, etc. Then, effects of different combinations between fluorophores and response groups on probe properties and detection performances are discussed.
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