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[Effect of ouabain on the Na+, K+-ATPase function and multiplication of transformed cells in culture]

N V Konobasova, Iu V Pashinin, O Iu Mel'nikova

    Tsitologiia
    |December 1, 1985
    PubMed

    Insights

    Ouabain affects cell proliferation and ATP hydrolysis in mouse hepatoma and L-cells. This drug

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Pharmacology

    Background:

    • Ouabain is a cardiac glycoside known to inhibit Na+, K+-ATPase.
    • Cell proliferation and ATP hydrolysis are fundamental cellular processes.
    • Understanding drug effects on these processes is crucial for various applications.

    Purpose of the Study:

    • To investigate the effect of ouabain on cell proliferation.
    • To examine the dependence of ATP hydrolysis on Na/K concentration in specific cell types.
    • To compare these effects in cells with varying sensitivities to ethidium bromide.

    Main Methods:

    • Studied ouabain's effect (10(-3) on cell proliferation.
    • Assessed ATP hydrolysis dependence on Na/K concentration in cell homogenates.
    • Utilized mouse hepatoma (XXIIa) and L-cells (sensitive and resistant to ethidium bromide).

    Main Results:

    • Na+, K+-ATPase activity was observed in cell homogenates even with ouabain present.
    • This activity was stimulated by Na/K ratios characteristic of dense cultures.
    • Ouabain's impact on cell proliferation mimicked the transition from sparse to dense cultures.

    Conclusions:

    • Ouabain influences Na+, K+-ATPase activity and cell proliferation.
    • The findings suggest a link between ouabain's effects and cellular density.
    • Further research can explore therapeutic strategies involving ouabain.

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