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Quantitative Analysis01:12

Quantitative Analysis

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Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
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Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Mobile Quantitative Colocalization Analysis of Fluorescence Microscopy Images.

Vadim Zinchuk1, Olga Grossenbacher-Zinchuk2

  • 1Department of Neurobiology and Anatomy, Kochi University Faculty of Medicine, Kochi, Japan.

Current Protocols in Cell Biology
|July 25, 2018
PubMed
Summary
This summary is machine-generated.

Quantifying fluorescent molecule colocalization is crucial. This new approach uses a cloud-synced system of desktop and mobile devices for seamless, convenient image analysis.

Keywords:
cloudfluorescence microscopyimage analysisquantitative colocalizationtablet computer

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Area of Science:

  • Microscopy and Image Analysis
  • Computational Biology
  • Biotechnology

Background:

  • Accurate quantification of colocalization in fluorescence microscopy is essential for understanding molecular interactions.
  • Current methods may lack accessibility and flexibility for researchers.
  • Mobile computing offers potential for enhanced analytical workflows.

Purpose of the Study:

  • To develop a novel, accessible approach for quantifying colocalization of fluorescent markers in microscopy images.
  • To integrate desktop and mobile computing platforms for seamless image analysis.
  • To improve the convenience and control of fluorescence microscopy data analysis.

Main Methods:

  • A three-component system involving a desktop computer, cloud storage, and a mobile device was developed.
  • Images are uploaded from a desktop to the cloud, making them accessible on a mobile device.
  • Software functionality is consistent across platforms, with seamless background synchronization via the cloud.

Main Results:

  • The developed system enables quantification of colocalization using both desktop and mobile platforms.
  • Image analysis states are synchronized across devices, ensuring data integrity.
  • The approach provides a seamless and user-friendly experience without requiring researcher input for synchronization.

Conclusions:

  • This novel approach significantly enhances accessibility to analytical imaging.
  • It offers superior control, simplicity, and convenience for fluorescence microscopy image analysis.
  • The integrated desktop-cloud-mobile system redefines workflows for researchers studying fluorescently-labelled molecules.