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Updated: Feb 7, 2026
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Imaging CD4 T Cell Interstitial Migration in the Inflamed Dermis
Published on: March 25, 2016
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Performance evaluation of an automated image-based fluorescence CD4+ cell analyzer.
Myoung-Ock Cho1, Subin Kim1, Ji Yeon Lee2
1Department of Mechanical Engineering, Graduate School, Kookmin University, Seoul 02707, Korea.
Summary
A new, cost-effective CD4+ cell counting analyzer, ADAMII, shows high correlation with conventional methods. This device offers a feasible solution for HIV/AIDS diagnosis in resource-limited settings.
Area of Science:
- Immunology
- Medical Diagnostics
- Biotechnology
Background:
- Antiretroviral therapy (ART) has reduced AIDS mortality, but HIV/AIDS remains prevalent in resource-limited regions.
- Developing countries often lack access to expensive diagnostic tools and skilled personnel for accurate CD4+ cell counting.
- Conventional flow cytometry for CD4+ cell counts is costly and requires specialized expertise, hindering widespread use.
Purpose of the Study:
- To develop and validate a cost-effective method for CD4+ cell counting.
- To provide an alternative to conventional flow cytometry for HIV/AIDS diagnosis in resource-limited areas.
- To enable accurate and accessible CD4+ cell enumeration for improved patient management.
Main Methods:
- Development of ADAMII, a benchtop fluorescence image-based CD3+/4+ cell counting analyzer.
- Utilizing a three-channel light source and an automatic 3D stage to capture up to 136 images.
- Image processing and analysis performed by integrated system software.
Main Results:
- ADAMII demonstrated a strong correlation (R2 = 0.98) when compared to FACSCalibur flow cytometry.
- ADAMII also showed a high correlation (R2 = 0.98) with the point-of-care PIMA CD4 analyzer.
- These results confirm the feasibility and reliability of CD4+ testing using ADAMII.
Conclusions:
- The ADAMII analyzer offers a practical and affordable solution for CD4+ cell counting.
- It can be readily implemented in resource-limited settings, replacing expensive and complex flow cytometers.
- This advancement facilitates improved HIV/AIDS monitoring and management in underserved populations.
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