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Updated: Feb 7, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Selective detection of chloramphenicol based on molecularly imprinted solid-phase extraction in seawater from
1Marine College, Shandong University, Weihai 264209, China.
Abstract:
This study highlights an efficient sample pre-treatment method for preconcentration and detection of chloramphenicol in marine water using molecularly imprinted solid-phase extraction (MISPE). Chloramphenicol molecularly imprinted microspheres were prepared and evaluated on the base of morphology, capacity and selectivity. The imprinted microspheres exhibited specific recognition and high retention capability to chloramphenicol and were applied as special solid-phase extraction adsorbents. An off-line MISPE protocol has been optimized and a creative analytical method coupled to HPLC-DAD was successfully developed for the cleanup and determination of chloramphenicol in seawater samples. Method performance was satisfactory with recoveries ranging from 81 to 90% and relative standard deviation (RSD) was <4.93% (n = 3). Accuracy of the method was assessed at three spiking concentration levels and the limit of detection was 5 ng L-1. Finally, five seawater samples from Jiaozhou Bay of China were determined and the results showed that there was no chloramphenicol detected.
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