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Updated: Feb 7, 2026

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
10:46

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

Published on: March 16, 2018

8.8K

Tumor Microenvironment-Response Calcium Phosphate Hybrid Nanoparticles Enhanced siRNAs Targeting Tumors InVivo

Zhihua Wu, Jianhua Chen, Ying Sun

    Journal of Biomedical Nanotechnology
    |July 26, 2018
    PubMed

    Insights

    This study developed an enzyme-responsive nanoparticle system for delivering short interfering RNA (siRNA) in cancer gene therapy. The novel siRNA@NP2 system effectively protects siRNA from degradation and enhances cellular uptake, showing promise for in vivo applications.

    Area of Science:

    • Biotechnology
    • Nanomedicine
    • Cancer Gene Therapy

    Background:

    • Short interfering RNA (siRNA) holds great promise for cancer gene therapy.
    • Current limitations include serum degradation, off-target effects, and poor cellular uptake of siRNA.

    Purpose of the Study:

    • To develop an enzyme-responsive hybrid delivery system for siRNA to overcome delivery challenges.
    • To evaluate the efficacy and safety of the novel siRNA delivery system in vitro and in vivo.

    Main Methods:

    • Construction of an enzyme-responsive PEG/Lipids/calcium phosphate hybrid nanoparticle system (siRNA@NP2).
    • Assessment of serum-induced degradation resistance and cellular uptake efficiency.
    • Evaluation of in vitro and in vivo delivery in SMMC-7721 cell lines and tumor-bearing nude mice.

    Main Results:

    • The siRNA@NP2 system demonstrated resistance to serum-induced degradation.
    • siRNA@NP2 exhibited efficient delivery in both cell lines and in vivo models.
    • Safety evaluations confirmed no significant toxicity of the nanoparticles in vitro and in vivo.

    Conclusions:

    • The developed enzyme-responsive nanoparticle system (siRNA@NP2) is an effective tool for siRNA delivery in cancer gene therapy.
    • This system overcomes key limitations of siRNA delivery, paving the way for improved in vivo therapeutic strategies.

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