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Chitinase: diversity, limitations, and trends in engineering for suitable applications
Ayokunmi Oyeleye1,2, Yahaya M Normi3,2
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia.
Bioscience Reports
|July 26, 2018
Summary
Chitinases break down chitin for industrial and agricultural uses. Protein engineering can overcome challenges in their application by enhancing specific features for improved efficiency.
Area of Science:
- Biochemistry and Biotechnology
- Enzymology
Background:
- Chitinases are enzymes that degrade chitin, a biopolymer found in fungi, crustaceans, and insects.
- These enzymes are valuable in medicine, agriculture, food, and environmental management due to their roles in producing useful products and controlling pests.
Purpose of the Study:
- To review the variations in chitinase features and mechanisms that limit biotechnological applications.
- To highlight recent advancements in engineering chitinases for enhanced efficiency.
Main Methods:
- Compilation of existing literature on chitinase diversity and limitations.
- Review of protein engineering strategies applied to chitinases.
Main Results:
- Chitinases exhibit diverse sources, characteristics, and mechanisms, complicating optimization and standardization for practical use.
- Inconsistencies between laboratory results and real-world applications are common.
Conclusions:
- Protein engineering offers a promising approach to overcome current limitations by modifying chitinases for specific applications.
- Further research into engineered chitinases is crucial for unlocking their full biotechnological potential.
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