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Updated: Feb 7, 2026

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Published on: January 25, 2017
Durable targeting of B-lymphocytes in living mice
M Cascalho1,2, D Huynh3, A R Lefferts3
1Department of Microbiology & Immunology, University of Michigan, 1150 W, Medical Center Dr., Ann Arbor, MI, 48109-5656, USA. marilia@umich.edu.
Abstract:
Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.
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