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Redox changes in coenzyme Q in the millisecond time range: an approach using rapid quenching and high-performance
Analytical Biochemistry
|November 15, 1985
Abstract:
We have combined a rapid-quenching protocol with HPLC analysis to measure the kinetics of reduction of coenzyme Q in a mitochondrial enzyme complex. The method has a time resolution of several milliseconds and will readily measure 1-20 nmol of the Q derivatives under investigation. By changing the HPLC solvent, either Q6 or Q10 can be studied.