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Updated: Feb 7, 2026

Labeling and Imaging Cells in the Zebrafish Hindbrain
Published on: July 25, 2010
NeuO for Neuronal Labeling in Zebrafish
Chai Lean Teoh1, Jun Cheng Er2, Parag Mukherjee3
1Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.
Abstract:
We report the application of our newly developed fluorescent probe 3-(benzylamino)-4,4-difluoro-5-(4-propyl-1H-1,2,3-triazol-1-yl)-8-(4-(2-hydroxyacetamido)phen-yl)-4-bora-3a,4a-diaza-s-indacene (NeuO) to label and image live neurons in zebrafish. Immersing zebrafish embryos in NeuO or injecting NeuO into zebrafish brain ventricles results in nontoxic in vivo neuronal labeling. We demonstrate the applicability of NeuO and envisage the potential of this compound as a rapid and simple labeling reagent for studying neuron development and degeneration.
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