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Related Experiment Video

Updated: Feb 7, 2026

Labeling and Imaging Cells in the Zebrafish Hindbrain

Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

NeuO for Neuronal Labeling in Zebrafish.

Chai Lean Teoh¹, Jun Cheng Er², Parag Mukherjee³

¹Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Singapore.

Tomography (Ann Arbor, Mich.)

|July 26, 2018

Summary

Researchers developed a new fluorescent probe, NeuO, for live neuron imaging in zebrafish. This nontoxic probe enables simple and rapid labeling for studying neuron development and degeneration.

Keywords:

BODIPY fluorescent probes imaging agents live neurons zebrafish

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Area of Science:

Neuroscience
Chemical Biology
Zebrafish Models

Background:

Accurate and efficient methods for live neuron imaging are crucial for understanding neural processes.
Existing labeling techniques may have limitations in terms of toxicity, speed, or simplicity.

Purpose of the Study:

To introduce and evaluate a novel fluorescent probe, NeuO, for in vivo neuronal labeling and imaging.
To assess the safety and efficacy of NeuO in live zebrafish models.

Main Methods:

Synthesis and characterization of the fluorescent probe NeuO.
Application of NeuO via immersion and injection into zebrafish embryos and larvae.
Microscopic imaging of labeled neurons in live zebrafish.

Main Results:

Successful and nontoxic labeling of live neurons in zebrafish using NeuO.
Demonstration of NeuO's applicability through both immersion and direct injection methods.
High-quality imaging of neuronal structures in vivo.

Conclusions:

NeuO is a versatile and effective fluorescent probe for live neuronal imaging in zebrafish.
The probe offers a rapid, simple, and nontoxic approach for visualizing neurons.
NeuO holds significant potential for research in neurodevelopment and neurodegeneration.