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Cor interacts with outer membrane proteins to exclude FhuA-dependent phages
Emma S Arguijo-Hernández1, Javier Hernandez-Sanchez1, Saida J Briones-Peña1
1Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 07360, Mexico City (CDMX), México.
Archives of Virology
|July 26, 2018
Summary
Cor, an outer membrane lipoprotein in Escherichia coli, prevents FhuA-dependent phage entry. It requires specific outer membrane proteins (OMPs) to mediate this superinfection exclusion (Sie) phenotype.
Area of Science:
- Microbiology
- Bacteriology
- Molecular Biology
Background:
- Superinfection exclusion (Sie) is a phage resistance mechanism.
- Cor protein in Escherichia coli mediates Sie against FhuA-dependent phages.
Purpose of the Study:
- To investigate the mechanism of Cor-mediated Sie.
- To identify outer membrane proteins (OMPs) involved in Cor function.
Main Methods:
- Western blot analysis to detect Cor species.
- Mutant strain construction and phenotypic analysis for Sie.
- Copurification affinity binding assays coupled with LC-ESI-MS/MS to identify protein interactions.
Main Results:
- Cor exists as preprolipoprotein/prolipoprotein and mature lipoprotein forms.
- Mutations in Cor affected the Sie phenotype.
- Cor directly binds to OMPs including OmpA, OmpC, OmpF, OmpW, LamB, and Slp.
- Cor-mediated Sie is dependent on specific OMPs, with reduced exclusion observed in knockout strains.
Conclusions:
- Cor is an outer membrane lipoprotein essential for FhuA-dependent phage exclusion.
- Cor requires interaction with specific OMPs to execute its Sie function.
- The findings elucidate a novel OMP-dependent mechanism for phage resistance in bacteria.
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