Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula
Myriam Sánchez1, Christine Le Signor1, Gregoire Aubert1
1INRA, University of Bourgogne Franche-Comté, Agroécologie, AgroSup Dijon, F-21000 Dijon, France.
Abstract:
TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18:455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest.
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