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Updated: Feb 7, 2026

Single-Molecule Imaging of Nuclear Transport
Published on: June 9, 2010
Single-molecule labeling for studying trafficking of renal transporters
Ankita Bachhawat Jaykumar1,2, Paulo S Caceres1, Pablo A Ortiz1,2
1Hypertension and Vascular Research Division, Department of Internal Medicine, Henry Ford Hospital , Detroit, Michigan.
Abstract:
The ability to detect and track single molecules presents the advantage of visualizing the complex behavior of transmembrane proteins with a time and space resolution that would otherwise be lost with traditional labeling and biochemical techniques. Development of new imaging probes has provided a robust method to study their trafficking and surface dynamics. This mini-review focuses on the current technology available for single-molecule labeling of transmembrane proteins, their advantages, and limitations. We also discuss the application of these techniques to the study of renal transporter trafficking in light of recent research.
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