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Diverse applications of TMB-based sensing probes
Li Huang1, Ying-Juan Cao, Xiang-Ying Sun
1College of Materials Science and Engineering, Huaqiao University, Xiamen 361021, China. jsshen@hqu.edu.cn.
New TMB derivatives, TMBS and TMBB, offer dual-mode sensing for pH, CO2, and glucose. These Schiff base probes provide fluorescence and UV-vis absorption signals, enabling practical applications in food and biological samples.
Area of Science:
- Analytical Chemistry
- Organic Synthesis
- Chemical Sensing
Background:
- 3,3',5,5'-tetramethylbenzidine (TMB) is a crucial enzyme substrate in analytical chemistry.
- TMB derivatives are valuable for developing novel analytical probes.
- Schiff base compounds offer versatile chemical reactivity and optical properties.
Purpose of the Study:
- Synthesize novel TMB derivatives (TMBS and TMBB) for enhanced analytical applications.
- Develop dual-modal (fluorescence and UV-vis absorption) sensing systems.
- Demonstrate the application of these probes for detecting pH, CO2, and glucose.
Main Methods:
- Facile one-step condensation reaction to synthesize TMBS and TMBB from TMB and aldehydes.
- Utilized the pH-dependent release of TMB from Schiff bases to generate optical signals.
- Investigated the influence of CO2 and glucose oxidase-catalyzed reactions on pH for sensing applications.
Main Results:
- Successfully synthesized TMBS and TMBB with dual-modal signal responses.
- Demonstrated practical pH sensing in real-world samples like rice vinegar and lemon juice.
- Established highly sensitive and selective dual-modal sensing systems for CO2 and β-d-glucose detection.
Conclusions:
- The synthesized TMB derivatives exhibit robust potential for versatile analytical applications.
- The dual-modal sensing approach offers enhanced detection capabilities.
- These probes provide a foundation for developing advanced chemical sensors.
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