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Study on Oxidation Stability and Oxygen Affinity of Hemoglobin During Storage
Qiuhui Li1, Huiya Ma1, Yuanyuan Zhang1
1College of Life Science, Northwest University, Xi'an, P.R. China.
Artificial Organs
|July 26, 2018
Summary
Highly deoxygenated hemoglobin (Hb) is best for long-term storage, as oxygenated Hb oxidizes quickly. Optimal in vitro conditions enhance Hb deoxygenation for developing oxygen carriers.
Area of Science:
- Biochemistry
- Biophysics
- Materials Science
Background:
- Hemoglobin (Hb) oxidation impacts its efficacy as an oxygen carrier.
- Methemoglobin (metHb) formation is a key indicator of Hb degradation during storage.
Purpose of the Study:
- To investigate factors influencing hemoglobin oxidation during short- and long-term storage.
- To determine optimal storage conditions for hemoglobin-based oxygen carriers.
Main Methods:
- Porcine Hb stored at 25°C and 4°C for 40 days with varying oxygen saturation.
- Accelerated stability testing at 37°C.
- Long-term stability testing at 4°C for 18 months.
- Assessment of temperature and pH effects on oxygen affinity (P50).
Main Results:
- Methemoglobin (metHb) content increased rapidly in half-saturated Hb at 4°C.
- Hb oxidation was more pronounced at 25°C compared to 4°C.
- Highly deoxygenated Hb showed minimal metHb increase at 37°C for 7 days, unlike oxygenated Hb.
- Highly deoxygenated Hb and polymerized hemoglobin remained stable at 4°C for 18 months.
- Increased temperature (20-40°C) and decreased pH (9 to 7) reduced Hb oxygen affinity, favoring deoxygenation.
Conclusions:
- Highly deoxygenated hemoglobin is suitable for both short- and long-term storage.
- Optimal in vitro conditions (high temperature, neutral pH) promote deoxygenation, crucial for hemoglobin-based oxygen carrier development.
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