Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K
From DNA to Protein03:06

From DNA to Protein

22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

14.8K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
14.8K
Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Molecule Visualization of DNase I-Mediated DNA Cleavage by High-Speed Atomic Force Microscopy.

ACS chemical biology·2026
Same author

Phage-encoded factor stimulates DNA degradation by the Hna anti-phage defense system.

Nature communications·2026
Same author

The evolving impact of virtual reality-based cultural immersion: A longitudinal mixed-methods study on EFL learners' intercultural sensitivity, willingness to communicate, and L2 learning motivation.

Acta psychologica·2026
Same author

Clinical validation and utility of targeted nanopore sequencing for rapid pathogen diagnosis and precision therapy in lung cancer patients with pulmonary infections.

Frontiers in cellular and infection microbiology·2026
Same author

The enzyme OsDHFR-TS1 controls leaf senescence during grain filling via folate-dependent chlorophyll biosynthesis in rice.

Plant physiology·2025
Same author

Phage-encoded factor stimulates DNA degradation by the Hna anti-phage defense system.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Feb 7, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

12.2K

Assessing Protein Dynamics on Low-Complexity Single-Stranded DNA Curtains.

Jeffrey M Schaub, Hongshan Zhang, Michael M Soniat

    Langmuir : the ACS Journal of Surfaces and Colloids
    |July 26, 2018
    PubMed
    Summary

    New single-molecule assays reveal how ssDNA-binding proteins (SSBs) compact flexible single-stranded DNA (ssDNA). E. coli SSB significantly alters ssDNA length, while RPA shows minimal changes, advancing DNA transaction research.

    More Related Videos

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
    08:53

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

    Published on: May 2, 2025

    998
    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
    06:32

    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

    Published on: March 9, 2022

    2.2K

    Related Experiment Videos

    Last Updated: Feb 7, 2026

    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
    10:23

    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

    Published on: May 8, 2015

    12.2K
    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
    08:53

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

    Published on: May 2, 2025

    998
    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
    06:32

    Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique

    Published on: March 9, 2022

    2.2K

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Biochemistry

    Background:

    • Single-stranded DNA (ssDNA) is crucial for DNA replication, repair, and transcription.
    • The flexibility of ssDNA means its length can be significantly altered by ssDNA-binding proteins (SSBs).
    • Understanding these interactions is key to elucidating DNA transaction mechanisms.

    Purpose of the Study:

    • To develop a high-throughput single-molecule assay for studying ssDNA-protein interactions.
    • To quantify the effects of SSBs on ssDNA length.
    • To investigate the binding of E. coli SSB and a modified RPA to ssDNA.

    Main Methods:

    • Development of a low-complexity ssDNA curtain assay on supported lipid bilayers.
    • Utilizing rolling circle replication for controlled ssDNA generation.
    • Employing biotin and fluorescent labeling for ssDNA tracking and length measurement.
    • High-throughput single-molecule imaging at microfabricated barriers.

    Main Results:

    • E. coli SSB was shown to drastically and reversibly compact ssDNA in response to NaCl concentration changes.
    • Interactions between a phosphomimetic RPA and ssDNA were examined.
    • RPA-ssDNA interactions were found to be largely unaffected by the tested modifications.

    Conclusions:

    • The developed ssDNA curtain assay is a powerful tool for single-molecule studies of ssDNA-binding proteins.
    • E. coli SSB significantly modulates ssDNA structure.
    • The assay provides insights into protein-DNA interactions relevant to DNA replication, transcription, and repair.