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Updated: Feb 7, 2026

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High Spatiotemporal Resolution Imaging with Localized Plasmonic Structured Illumination Microscopy.

Anna Bezryadina1, Junxiang Zhao1, Yang Xia2

  • 1Department of Electrical and Computer Engineering , University of California, San Diego , La Jolla , California 92093 , United States.

ACS Nano
|July 26, 2018
PubMed
Summary

Localized plasmonic structured illumination microscopy (LPSIM) achieves 50 nm resolution for multicolor live-cell imaging at video rates. This high-speed, low-phototoxicity technique enables detailed observation of protein dynamics and interactions.

Keywords:
LPSIMlive-cell imagingnanofabricationplasmonicsstructure illumination microscopysuper-resolution microscopy

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Traditional microscopy is limited by the diffraction of light, hindering nanoscale resolution.
  • Super-resolution microscopy techniques offer enhanced resolution but often face challenges with speed, phototoxicity, or complex sample preparation.
  • Localized plasmonic structured illumination microscopy (LPSIM) has emerged as a promising approach to overcome these limitations.

Purpose of the Study:

  • To demonstrate a wide-field LPSIM system capable of achieving high spatial resolution at video rate speeds.
  • To evaluate the system's performance in imaging dynamic biological processes with low phototoxicity.
  • To assess the suitability of LPSIM for routine biological sample imaging.

Main Methods:

  • Utilized a nanoscale plasmonic antenna array to generate tunable, sub-diffraction illumination patterns.
  • Implemented a wide-field imaging configuration for simultaneous multicolor super-resolution data acquisition.
  • Applied LPSIM to image microtubule dynamics in live cells using low illumination power intensity.

Main Results:

  • Achieved a spatial resolution of 50 nm with the wide-field LPSIM system.
  • Demonstrated high-speed imaging capability, reaching video rate speeds.
  • Successfully imaged dynamic biological structures (microtubules) with minimal phototoxicity.

Conclusions:

  • LPSIM offers a powerful tool for multicolor, wide-field super-resolution live-cell imaging with high speed and low phototoxicity.
  • The system's design is compatible with standard sample preparation, facilitating broader application.
  • LPSIM holds significant potential for advancing the study of protein dynamics and interactions in live cells.