Related Experiment Video

Updated: Feb 7, 2026

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

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Disulfiram reduces metastatic osteosarcoma tumor burden in an immunocompetent Balb/c or-thotopic mouse model

Jared Anthony Crasto1, Mitchell Stephen Fourman1, Alejandro Morales-Restrepo1

  • 1Musculoskeletal Oncology Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.

Oncotarget
|July 27, 2018
PubMed
Abstract

Insights

Disulfiram significantly reduced osteosarcoma metastasis in mice, matching doxorubicin efficacy. Combining disulfiram with doxorubicin did not improve outcomes compared to doxorubicin alone.

Area of Science:

  • Oncology
  • Pharmacology
  • Cancer Metastasis Research

Background:

  • Osteosarcoma (OS) with pulmonary metastases has a poor survival rate (15-30%).
  • Disulfiram (DSF), an aldehyde dehydrogenase inhibitor, shows in vitro efficacy against OS metastasis.
  • Current treatments for metastatic OS have limited success.

Purpose of the Study:

  • To evaluate the in vivo efficacy of disulfiram (DSF) in reducing osteosarcoma (OS) metastasis.
  • To compare DSF's efficacy against doxorubicin (DXR) chemotherapy in a validated orthotopic OS model.
  • To assess the potential additive effect of combining DSF and DXR.

Main Methods:

  • 120 immunocompetent mice received K7M2 murine OS cells.
  • Treatments included saline, low-dose DSF, high-dose DSF, DXR, and combinations.
  • Metastatic tumor burden was quantified using near-infrared indocyanine green (ICG) angiography.

Main Results:

  • All treatment groups showed significantly reduced OS metastatic burden compared to controls.
  • Low-dose DSF treatment was equivalent in efficacy to doxorubicin (DXR).
  • 95% of control animals had metastatic disease, significantly higher than all treatment groups.

Conclusions:

  • Disulfiram treatment effectively reduced osteosarcoma metastatic burden in vivo.
  • DSF demonstrated comparable efficacy to doxorubicin in this model.
  • No additive therapeutic benefit was observed when combining disulfiram and doxorubicin.
Keywords:
Aktaldehyde dehydrogenasebaddisulfiramosteosarcoma

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