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Updated: Feb 7, 2026

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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MMP-13 binds to platelet receptors αIIbβ3 and GPVI and impairs aggregation and thrombus formation

Joanna-Marie Howes1, Nicholas Pugh2, Samir W Hamaia1

  • 1Department of Biochemistry University of Cambridge Cambridge UK.

Research and Practice in Thrombosis and Haemostasis
|July 27, 2018
PubMed

Insights

Matrix metalloproteinase-13 (MMP-13) inhibits platelet aggregation and thrombus formation by binding to platelet receptors. This discovery offers new insights into atherothrombotic pathologies.

Area of Science:

  • Cardiovascular Biology
  • Thrombosis Research
  • Molecular Medicine

Background:

  • Atherosclerotic plaque rupture triggers thrombus formation, leading to myocardial infarction and stroke.
  • Collagen exposure during plaque rupture activates platelets, initiating aggregation.
  • Other plaque components can influence platelet function and thrombus development.

Purpose of the Study:

  • To investigate the role of matrix metalloproteinase-13 (MMP-13) in platelet aggregation and thrombus formation.
  • To determine if MMP-13, upregulated in atherothrombotic conditions, impacts platelet function.

Main Methods:

  • Assessed MMP-13 binding to platelet receptors alphaIIbbeta3 (αIIbβ3) and glycoprotein (GP)VI.
  • Evaluated the effect of MMP-13 on washed platelet aggregation.
  • Measured thrombus formation on fibrillar collagen in flowing blood.

Main Results:

  • MMP-13 binds to platelet receptors αIIbβ3 and GPVI.
  • MMP-13 significantly inhibits washed platelet aggregation.
  • MMP-13 decreases thrombus formation on fibrillar collagen.

Conclusions:

  • MMP-13 inhibits platelet aggregation and thrombus formation in flowing blood.
  • MMP-13 may play a subtle role in atherothrombotic pathologies.
  • Findings suggest new research avenues for understanding MMP-13's mechanisms in cardiovascular disease.
Abstract

Keywords:
GPVI collagen receptorIntegrin alphaIIbbeta3 (αIIbβ3)matrix metalloproteinase‐13plateletsthrombosis

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