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Updated: Feb 7, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Laboratory Culturing Techniques and Maintenance of Vibrio cholerae
Kyle D Brumfield1, Bailey M Carignan1, Mike S Son2
1Department of Biological Sciences, Plymouth State University, Plymouth, NH, USA.
Abstract:
Cholera is a severe diarrheal disease caused by the consumption of food or water contaminated with the aquatic gram-negative bacterium Vibrio cholerae. Infected hosts will experience vomiting and severe watery diarrhea and if not treated properly will ultimately succumb to death by dehydration. Due to the global prevalence and severity of cholera, V. cholerae has been extensively studied in both environmental and laboratory settings. Herein, we describe proper V. cholerae maintenance, in addition to classical and El Tor biotype culturing in a laboratory setting.
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