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Updated: Feb 7, 2026

Vibrio cholerae: Model Organism to Study Bacterial Pathogenesis - Interview
Published on: May 28, 2007
Random Transposon Mutagenesis of Vibrio cholerae
1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA. Jyl.Matson@utoledo.edu.
Abstract:
Transposon-based random mutagenesis of bacterial genomes has proven to be a powerful genetic tool for the identification of genes and regulatory elements that contribute to specific phenotypes. One such approach that has been used in Vibrio cholerae for many years is the introduction of mariner transposons to generate random libraries of mutants. These libraries have been successfully used for a wide variety of genetic screens and selections in this important bacterial pathogen. Here we present a detailed protocol for the use of plasmid pFD1 (containing the mariner transposon magellan3) to create mutant libraries in V. cholerae.
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