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A sensitive resonance Rayleigh light scattering method for alpinetin using gold nanorods probes
Shuyun Bi1, Xiaoyue Sun1, Yu Wang1
1College of Chemistry, Changchun Normal University, Changchun, Peoples Republic of China.
Abstract:
A sensitive resonance Rayleigh light scattering (RLS) assay for alpinetin was developed based on alpinetin-modified gold nanorods (AuNRs). Alpinetin could interact with AuNRs and formed a new assembly by electrostatic attraction. In pH 7.4 Tris-HCl buffer solution, the assembly of alpinetin-AuNRs showed a sensitive RLS signal. Under optimum conditions, the magnitude of enhanced RLS intensity (ΔIRLS ) was proportional to the concentration of alpinetin over the range 0.027-3.24 μg ml-1 , with a detection limit of 1.79 ng ml-1 (by 3σ). The developed RLS method was successfully applied to the detection of alpinetin in real or synthesized samples. Alpinetin recoveries were 90.4-108.7% with an RSD of 0.82-2.9% (n = 5) for Alpinia katsumadai samples, and 95.1-103.7% with an RSD of 0.28-3.9% (n = 5) for synthesized samples. The results showed that this new approach was convenient, reliable and sensitive.
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