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Updated: Feb 7, 2026
12:09
Methods for Intravenous Self Administration in a Mouse Model
Published on: December 8, 2012
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Intravenous Administration-Oriented Pharmacokinetic Model for Dynamic Bioluminescence Imaging.
IEEE Transactions on Bio-Medical Engineering
|July 27, 2018
Summary
This study introduces a pharmacokinetic (PK) model for quantitative in vivo bioluminescence imaging (BLI) using intravenous D-luciferin injection. The model predicts tumor growth earlier and more sensitively than conventional methods.
Area of Science:
- Biomedical Imaging
- Pharmacokinetics
- Oncology
Background:
- In vivo bioluminescence imaging (BLI) is valuable for tumor monitoring but quantitative applications using intravenous (IV) injection are limited.
- Existing methods restrict the broader application of BLI in tumor growth and metastasis studies.
Purpose of the Study:
- To develop and validate a pharmacokinetic (PK) model for quantitative BLI following IV administration of D-luciferin.
- To enhance the sensitivity and early detection of tumor growth and metastasis using dynamic BLI.
Main Methods:
- Designed a PK model for IV D-luciferin administration in mkn28-luc xenografted mice.
- Acquired dynamic BLI data over 40 minutes post-injection on multiple days.
- Applied the PK model to dynamic BLI data to calculate the sum of kinetic rate constants (SKRC).
Main Results:
- SKRC values demonstrated a rapid decrease correlating with tumor growth.
- Statistically significant differences in SKRC were observed across different time points.
- The time point of peak luminous intensity remained unaffected by tumor progression.
Conclusions:
- Dynamic BLI combined with the developed PK model enables earlier and more sensitive prediction of tumor growth.
- This approach is crucial for improving the efficacy of drug evaluation in preclinical studies.
- Dynamic BLI may offer a noninvasive method for acquiring arterial input function, advancing hybrid PET-optical imaging.
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