Related Experiment Video
Updated: Feb 7, 2026
10:00
Preparation of CD4+ T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy
Published on: November 8, 2016
9.0K
Aberrantly expressed lncRNAs identified by microarray analysis in CD4+T cells in asthmatic patients
Xuefei Qi1, Huilong Chen2, Zhenli Huang1
1Department of Respiratory and Critical Care Medicine, Key Laboratory of Pulmonary Diseases of Health Ministry, Key Cite of National Clinical Research Center for Respiratory Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Sciences & Technology, Wuhan, China.
Biochemical and Biophysical Research Communications
|July 28, 2018
Summary
Researchers identified distinct long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in CD4+ T cells from asthma patients. These findings suggest potential new biomarkers for asthma diagnosis and treatment.
Area of Science:
- Immunology
- Genetics
- Respiratory Medicine
Background:
- Asthma is a chronic respiratory disease characterized by airway inflammation.
- CD4+ T cells play a crucial role in the immune response during asthma.
- Understanding molecular changes in CD4+ T cells is vital for asthma research.
Purpose of the Study:
- To profile messenger RNA (mRNA) and long non-coding RNA (lncRNA) expression in CD4+ T cells of asthma patients.
- To investigate the clinical value and biological functions of differentially expressed lncRNAs.
- To identify potential diagnostic biomarkers for asthma.
Main Methods:
- Microarray analysis was used to compare gene expression profiles in CD4+ T cells from asthma patients and healthy controls.
- Quantitative real-time polymerase chain reaction (qRT-PCR) validated the expression of specific lncRNAs.
- Bioinformatics analyses were performed to elucidate the functional roles of lncRNAs.
Main Results:
- A significant number of lncRNAs (2725) and mRNAs (3167) were found to be differentially expressed.
- Three lncRNAs were upregulated and one was downregulated, with high diagnostic accuracy shown by receiver operating characteristic curve analysis.
- Specific lncRNAs correlated with clinical parameters like IL-13 levels and peak expiratory flow.
Conclusions:
- Aberrantly expressed lncRNAs in CD4+ T cells are implicated in asthma pathogenesis.
- These lncRNAs hold potential as novel biomarkers for asthma diagnosis.
- Further research into the biological functions of these lncRNAs could reveal therapeutic targets.
Related Concept Videos
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
02:34DNA Microarrays
21.2K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
21.2K
01:42What is Gene Expression?
196.9K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
01:36What is Gene Expression?
11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
01:14Identifying Statistically Significant Differences: The F-Test
3.8K
The F-test is used to compare two sample variances to each other or compare the sample variance to the population variance. It is used to decide whether an indeterminate error can explain the difference in their values. The underlying assumptions that allow the use of the F-test include the data set or sets are normally distributed, and the data sets are independent of each other. The test statistic F is calculated by dividing one variance by another. In other words, the square of one standard...
3.8K