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High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated
Stefano Dugheri1, Alessandro Bonari2, Matteo Gentili3
1Laboratorio di Igiene e Tossicologia Industriale, Azienda Ospedaliero-Universitaria Careggi, Largo P. Palagi 1, 50139 Firenze, Italy. stefano.dugheri@unifi.it.
Molecules (Basel, Switzerland)
|July 28, 2018
Summary
A new user-friendly platform for detecting occupational exposure biomarkers using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry offers accurate and precise results. This method aids in developing effective occupational hygiene strategies.
Area of Science:
- Analytical Chemistry
- Occupational Health
- Environmental Science
Background:
- High-throughput screening is crucial for detecting occupational exposure biomarkers.
- Existing methods require user-friendly, automated apparatus for prompt, selective, precise, and accurate results.
- Miniaturization in analytical chemistry offers sample and solvent savings and easier automation.
Purpose of the Study:
- To develop a novel, user-friendly solid-phase microextraction (SPME) platform for occupational exposure biomarker detection.
- To couple the SPME platform with gas chromatography and triple quadrupole-mass spectrometry for analyzing urinary polycyclic aromatic hydrocarbon (PAH) metabolites.
- To provide a sensitive, accurate, and precise method for monitoring exposure to specific OH-PAHs.
Main Methods:
- Development of a user-friendly off- and on-line SPME platform.
- Coupling SPME with gas chromatography and triple quadrupole-mass spectrometry.
- Utilizing direct immersion sampling with on-fiber derivatization using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide.
- Employing specific isotope-labelled internal standards for accurate quantification.
Main Results:
- The method successfully determined seven urinary OH-PAHs: 1- and 2-hydroxy-naphthalene, 9-hydroxy-phenanthrene, 1-hydroxy-pyrene, 3- and 9-hydroxy-benzoantracene, and 3-hydroxy-benzo[a]pyrene.
- Detection limits for the seven OH-PAHs ranged from 0.25 to 4.52 ng/L.
- Excellent repeatability was achieved, with intra-session ranging from 2.5% to 3.0% and inter-session from 2.4% to 3.9%.
Conclusions:
- The developed SPME-GC-MS/MS platform is a user-friendly and efficient tool for occupational exposure biomarker analysis.
- The method's sensitivity, precision, and accuracy support its application in occupational hygiene programs.
- This technology can help identify effective risk-control strategies for workplace safety.
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