Alpha 2 adrenoceptors modulate the nociceptive jaw-opening reflex in rats

The jaw-opening reflex in lightly anesthetized rats induced by intrapulpal (left maxillary) electrical tooth pulp stimulation and quantified by the electromyograms (threshold values) recorded from the ipsilateral digastric muscle was used as the experimental pain index. The threshold for the jaw-opening reflex was significantly elevated by clonidine (12.5 to 50 micrograms/kg, i.v.) and was inversely correlated with the frequency of stimulation. The analgesia elicited by clonidine was antagonized by pretreatment with the alpha 2-adrenoceptor antagonist yohimbine (1 mg/kg, i.v.). All doses of clonidine produced an initial transient pressor response followed by a sustained hypotension and bradycardia. However, there was no direct correlation between the antinociceptive and cardiovascular effects of clonidine. It is proposed that clonidine modulates jaw-opening reflex analgesia by specifically activating alpha 2-adrenoceptors.