Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microsomal membrane subfractionation by a lectin affinity method.

D Sehmi, L G Williams, D J Williams

    FEBS Letters
    |March 3, 1986
    PubMed
    Summary

    Concanavalin A-agarose effectively isolates plasma membrane fragments from rat liver microsomes. This method removes cholesterol-rich components and cell surface radiolabels, aiding in microsomal membrane subfractionation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Statement on the Importance of Sexuality and Gender Research.

    Journal of sex research·2025
    Same author

    Defence context for the UK's Defence Engagement (Health).

    BMJ military health·2023
    Same author

    Current biopsychosocial science on understanding kink.

    Current opinion in psychology·2022
    Same author

    Comparison of the Respiratory Resistomes and Microbiota in Children Receiving Short versus Standard Course Treatment for Community-Acquired Pneumonia.

    mBio·2022
    Same author

    Emergency hip disarticulations for severe necrotising fasciitis of the lower limb: a series of rare cases from a rural district general hospital.

    Annals of the Royal College of Surgeons of England·2021
    Same author

    Mass violence planned as ultimate leisure experience? Four diverse cases.

    Journal of forensic sciences·2021

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biology

    Background:

    • Microsomal fractions contain membrane fragments from various cellular organelles.
    • Subfractionation of microsomes is crucial for studying specific membrane components.
    • Rat liver microsomes are a common model for studying membrane composition and function.

    Purpose of the Study:

    • To investigate the utility of Concanavalin A-agarose for isolating plasma membrane fragments from rat liver microsomes.
    • To characterize the biochemical properties of the fraction removed by Concanavalin A-agarose.
    • To assess the potential of this method for microsomal membrane subfractionation.

    Main Methods:

    • Treatment of rat liver post-mitochondrial supernatant with Concanavalin A-agarose.
    • Analysis of the removed fraction for cholesterol content and enzyme activities (5'-nucleotidase, glucose-6-phosphatase).
    • Assessment of radiolabel removal associated with the cell surface.

    Main Results:

    • Concanavalin A-agarose removed a fraction enriched in cholesterol and 5'-nucleotidase activity.
    • This fraction was depleted of glucose-6-phosphatase activity.
    • Cell surface-associated radiolabel was effectively removed, indicating plasma membrane association.

    Conclusions:

    • Concanavalin A binds to and removes plasma membrane fragments from the microsomal fraction.
    • This technique offers a gentle and rapid method for subfractionating microsomal membranes.
    • The findings suggest Concanavalin A-agarose is a valuable tool for isolating specific membrane components.

    Related Experiment Videos