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High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

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Published on: August 18, 2023

Establishing a Cell-Based High-Content Screening Assay for TCM Compounds with Anti-Renal Fibrosis Effects.

Xi-Ting Wang¹, Xue-Jiao Sun¹, Cheng Li¹

¹School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.

Evidence-Based Complementary and Alternative Medicine : Ecam

|July 28, 2018

Summary

This study developed a high-content screening (HCS) method to find traditional Chinese medicine (TCM) compounds that fight renal fibrosis. Eight effective compounds were identified, offering new avenues for chronic kidney disease treatment.

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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

Area of Science:

Nephrology
Pharmacology
Drug Discovery

Background:

Renal fibrosis is a key factor in chronic kidney disease (CKD) progression, with limited treatment options beyond renal replacement therapy.
Traditional Chinese Medicine (TCM) offers a promising source for novel antifibrotic compounds.
Developing effective screening methods is crucial for identifying these compounds.

Purpose of the Study:

To establish a robust cell-based high-content screening (HCS) assay for identifying TCM compounds with antifibrotic properties.
To screen a library of 344 TCM molecules for potential anti-renal fibrosis activity.
To validate identified compounds and explore novel therapeutic agents for CKD.

Main Methods:

Optimized experimental parameters for a stable and reproducible NRK49F cell-based model.
Validated the HCS model using a known TGF-β1 receptor inhibitor (SB525334) and established TCM antifibrotics (curcumin, emodin).
Screened a library of 344 TCM compounds using the validated HCS assay and performed verification experiments on positive hits.

Main Results:

A high-content screening (HCS) model was successfully developed and validated for identifying anti-renal fibrosis compounds.
Out of 344 TCM molecules screened, 16 showed prospective inhibitory activity.
Eight compounds demonstrated significant anti-renal fibrosis effects, including six novel agents: Ligustrazine, Glycyrrhizic acid, Astragaloside iv, Hydroxysafflor Yellow A, Crocin, and Gypenosides.

Conclusions:

The developed HCS assay is a reliable and effective tool for discovering TCM compounds with anti-renal fibrosis activity.
This study identified several promising compounds, including novel agents, for the potential treatment of renal fibrosis and CKD.
The HCS approach can be broadly applied to identify lead compounds from TCM for various diseases.