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Current trends in supercritical fluid chromatography.

Caroline West1

  • 1ICOA, University of Orleans, CNRS UMR 7311, Rue de Chartres, BP 6759, 45067, Orléans, France. caroline.west@univ-orleans.fr.

Analytical and Bioanalytical Chemistry
|July 28, 2018
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Summary

Supercritical fluid chromatography (SFC) is experiencing a revival due to modern instruments and techniques like ultra-high-performance SFC (UHPSFC). Applications in natural products, bioanalysis, food, and environmental science are growing rapidly.

Keywords:
Convergence chromatographyHyphenation to mass spectrometrySupercritical fluid chromatographyUltra-high-performance supercritical fluid chromatography

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Separation Science

Background:

  • Supercritical fluid chromatography (SFC) uses pressurized carbon dioxide as a mobile phase.
  • Modern instrumentation has led to increased interest in SFC.
  • SFC offers advantages over traditional liquid chromatography.

Purpose of the Study:

  • To review recent trends in supercritical fluid chromatography (SFC) applications.
  • To identify shifts in SFC application domains based on recent literature.
  • To highlight advancements in SFC instrumentation and techniques.

Main Methods:

  • Review of approximately 500 peer-reviewed papers published between 2014 and 2018.
  • Analysis of trends in application domains, instrumentation, and hyphenation.
  • Comparison of recent SFC developments with older literature.

Main Results:

  • Significant increase in SFC applications for natural products, bioanalysis, food science, and environmental analyses.
  • Development of ultra-high-performance SFC (UHPSFC) using sub-2-μm and superficially porous particles for improved resolution and speed.
  • Increased hyphenation of SFC with mass spectrometry, opening new application areas.
  • Blurring of boundaries between SFC and HPLC with modern instruments facilitating method switching.

Conclusions:

  • SFC is undergoing a resurgence driven by technological advancements.
  • UHPSFC and hyphenation with mass spectrometry are key emerging trends.
  • SFC is becoming more versatile and integrated with other chromatographic techniques.