[Assessment on Application of 24 Y-STR Loci in Forensic Science]

Area of Science:

• Forensic genetics
• Population genetics
• Molecular biology

Background:

• Selecting effective Y-STR marker systems is crucial for forensic identification.
• Assessing mutation rates and haplotype diversity is essential for evaluating forensic applicability.

Purpose of the Study:

• To identify a Y-STR marker system with superior haplotype identification capabilities.
• To evaluate the mutation rate and forensic application potential of a selected Y-STR system.

Main Methods:

• A self-developed 24 Y-STR fluorescent multiplex system was utilized.
• Forensic assessment involved 139 father-son sample pairs from Jinan, Shandong.

Main Results:

• The 24 Y-STR loci identified 176 alleles with gene diversity ranging from 0.0837 to 0.9669.
• 139 unique haplotypes were observed in 139 unrelated males, yielding a haplotype diversity of 1 and discrimination capacity of 1.
• Five one-step mutation events were detected, resulting in an average mutation rate of 0.0015.

Conclusions:

• The 24 Y-STR loci system exhibits robust individual identification power in the Jinan, Shandong Han population.
• The system's low mutation rate and high discrimination capacity indicate significant value in forensic science.