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Updated: Feb 7, 2026

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
Published on: April 20, 2015
Anthrax protective antigen is a calcium-dependent serine protease
Lisanne Storm1, Floris J Bikker2, Kamran Nazmi2
1a Department of Medical Microbiology and Infectious Diseases , Erasmus University Medical Centre , Rotterdam , The Netherlands.
Abstract:
Bacillus anthracis secretes a three component exotoxin-complex, which contributes to anthrax pathogenesis. Formation of this complex starts with the binding of protective antigen (PA) to its cellular receptor. In this study, we report that PA is a calcium-dependent serine protease and that the protein potentially uses this proteolytic activity for receptor binding. Additionally our findings shed new light on previous research describing the inhibition of anthrax toxins and exotoxin formation. Importantly, inhibition of the proteolytic activity of protective antigen could be a novel therapeutic strategy in fighting B. anthracis-related infections.
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