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Structural comparisons between mouse and human prealbumin.

S Wakasugi, S Maeda, K Shimada

Journal of Biochemistry

|December 1, 1985

Summary

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Researchers cloned mouse prealbumin cDNA to create models for familial amyloidotic polyneuropathy. This mouse prealbumin shares structural similarities with human prealbumin, aiding in amyloidosis research.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Familial amyloidotic polyneuropathy (FAP) is a debilitating disease.
Mouse models are crucial for understanding and treating human diseases.

Purpose of the Study:

To clone mouse prealbumin cDNA for developing FAP model systems.
To analyze the structural differences between mouse and human prealbumin.

Main Methods:

Cloning mouse prealbumin cDNA using human prealbumin cDNA as a probe.
Deducing primary protein structure from cDNA sequence analysis.

Main Results:

Mouse prealbumin consists of 147 amino acids (127 mature, 20 signal sequence), similar to human prealbumin.

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25 amino acid substitutions were found in mouse prealbumin compared to human.

Most substitutions are on the protein's surface, with conserved core and channel regions.

Conclusions:

The cloned mouse prealbumin cDNA is valuable for creating FAP models.
Structural conservation suggests functional similarities relevant to amyloidosis research.